CURRICULUM VITAE

Manuela Filippini, PhD

Email: manuela(dot)filippini(at)gmail(dot)com

I am looking for an interesting position as
postdoc, research assistant, technical assistant


University of Zurich
Institute of Evolutionary Biology & Environmental Studies
Evolutionary Genetics and Theoretical Biology



Citizenship:
Swiss
Education:
2004-2007 PhD Student: (Thesis defended on the 13th of April 2007)
Structure and dynamics of viral communities in aquatic ecosystems
Prof. Dr. Mark Gessner, Eawag/ETH, Switzerland &
Prof. Dr. Mathias Middelboe, MBL, Denmark

2001 graduation as Diplom Botanist
MSc thesis: "Cause of the collaps of the Planktothrix rubescens population in Lake Zurich
(in German)

Tutor: Prof. Dr. F. Schanz,Limnological Station, University of Zurich, Kilchberg
Supervisor: Prof. Dr. F. Jüttner, Limnological Station, University of Zurich, Kilchberg
Professional Experiences:

* Aug 07- Mai 12 Postdoc University Zurich, Evolutionary Genetics and Theoretical Biology,
Supervisor: Prof. Dr. H. Bagheri
* Jun-Sept 2007 Postdoc EAWAG, Aquatic Ecology, Kastanienbaum,
Supervisor: Prof. Dr. M. Gessner
* 2002-2004 Research Assistant at the Limnological Research Center of Eawag, Department of Limnology, Group Prof. Dr. M. Gessner
* 2002 Guest at the University of Clermont Ferrand, France, to analyse viruses in natural samples by Transmission Electron Microscopy (Dr. Sime-Ngando T. and Dr. Bettarel Y.)
* 1999 (3 months) Assistant at the Limnological Research Center, Eawag, in preparation of and during Symposium of the Group of Aquatic Production (GAP) of the International Society of Limnology (SIL)

Teaching:

2007, 08 & 09 Assistant during the 6 weeks course in Principles of Evolution, University of Zurich
2006 & 02 Assistant in a practical course in Aquatic Ecology of ETH Zurich for biology students
2000 Assistant in various Limnology courses at University of Zurich for environmental science students

Specific laboratory skills:

• Aseptic technique

Isolation, purification and storage of bacteria
Characterization of bacteria using a polyphasic approach
(phenotypic, chemotaxonomic and phylogenetic)
Genome sequencing (454 pyrosequencing)

• Epifluorescence microscopy (EM), transmission electron microscopy (TEM)

Bacterial production, abundance, morphology (3H-Leu, SybrGold, Live/dead BacLight)
Bacterial community structure (PCR, DGGE, Cloning, Sequencing; RLFP)
Infection rate of bacteria (viral mortality of bacteria, TEM-Analysis)

Viral production, abundance, morphology (SybrGold,EM,TEM, FC)
Viral community structure (PFGE, PCR-DGGE)

Protein isolation, semi purification and SDS-PAGE, Zymogram for activity

Competition and evolution experiments

Cellulose and lignin content of organic matter (acid-detergent fibre method after Goering & Van
Soest), Lignin content of organic matter (alkaline CuO oxidation method after Goni & Montgomery)

Pigment isolation, spectrum

Continuing education courses:

2012 BSO biosafety level 2, b-safe, Bellinzona
2011
GMP in Pharmaceuticals industry, Aprentas, Basel
2011
Basic Course Cell Culture, LAB-ACADEMY, Taufkirchen, Germany
2011 Basic Course Immunology, LAB-ACADEMY, Taufkirchen, Germany
2011 BSO biosafety level 1, b-safe, Bern
2011 Advanced subject-specific training: GMP and Biosafety, b-safe, Bern
2011 Safety work in a biosafety labor 2 and 3 (BSL2, BSL3), b-safe, Bern
2010 Foundations of Modelling and Scientific Computing in Biology, University of Zurich
2009 Bacterial Proteomics, 5 days Training Course, PD Dr. K Riedel, University of Zurich & Functional Genomics Center Zurich
2007 A Primer on Molecular Evolution and Phylogeny, University of Cambridge, UK
2006 Practical workshop on Virus ecology methods, Marine Biological Association of the UK, Plymouth, UK
2006 Safety Awareness, Fire Fighting and Sea rescue, STMS Survival Training Maritime Safety, Esbjerg, Denmark
2006
Virology course, UNI ZH
2004
Introduction to the Flow Cytometer, UNI Bern
2004 Writing English for Science, Dübendorf ZH
2003 Course in "Effects of changes in climate on arctic, sub-arctic and temperate freshwater,
Monsted Field Station, Denmark
2003 Ecotoxicology course, (Risk Assessment), Dübendorf ZH
2002 Biology and Ecology of marine Algae, Seminar in Roscoff, France, organized by University of Zurich, Prof. F. Schanz
2002 Basis of Toxicology, UNI Zürich,
2002 Ecotoxicology course, (Impairment of natural systems), Syngenta AG Basel,
2002 Advanced Course in Molecular Microbial Ecology at the Alpine Center Piora (Tessin, Switzerland), R.Amann und R.Bachofen
2002 Cycle Postgrade in Environmental Science EPF Lausanne, Module 6: Management of an ecosystem
(Basis of ecotoxocology)
2001 Ecotoxicology course EPF Lausanne
2001 Seeplankton (Diatoms) in Dübendorf(ZH)

Languages:

Italian (mother tongue); French and German fluently; English good and Spanish knowledges

Computer skills:

Applications: Windows XP, MSOffice, GoLive, Adobe Acrobat, Adobe Illustrator
Phylogenetic programs: Geneious, Clustal_X, MEGA, Paup, Garli, Mauve
Statistical programs: SPSS

Scientific expedition:
2009 (March), University Zurich, North Sea, Cyanobacteria Diversity
2007 (February-May) Danish scientific expedition, Galathea 3,
Geomicrobiology of oceanic oxygen deficiency zones (GOODZ)
Collaborations:

Dr. T.H.M Smits, Agroscope Changins-Wädenswill, Switzerland
PD. Dr. H. Brandl, Institute of Evolutionary Biology and Environmental Studies, Uni Zurich
Dr. W. Qi, UZH/ ETH Functional Genomics Center Zurich, Zurich, Switzerland
Dr. M. Middelboe, Marine Biology Laboratory, Helsingoer , Denmark
Dr. B. Wrobel, Evolutionary Systems Laboratory IOPAN & UAM, Poznań , Poland
Dr. U. Ziegler & A. Keach, UZH Center for Microscopy and Image Analysis, Zurich, Switzerland
Dr. E. Laczko, UZH/ETH Functional Genomics Center Zurich, Zurich, Switzerland
Dr. S. Jacquet, Station d’Hydrobiologie Lacustre, Equipe de Microbiologie Aquatique, INRA, Thonon les Bains
Dr. I. Domaizon, Université de Savoie, UMR CARRTEL, Bourget-du-lac, France
Dr. C. Schubert, EAWAG/ETH Department of Surface Waters, Kastanienbaum, Switzerland
Dr. T. Sime-Ngando & Dr. Y. Bettarel, Laboratoire de Biologie des Protistes, Clermont Ferrand, France
Dr. N. Buesing, Eawag, Department of Limnology, Kastanienbaum, Switzerland
Dr. H. Buergmann, Institute of Terrestrial Ecology, ETH Zurich, Schlieren,
present adress Department of Surface Waters, Kastanienbaum, Switzerland


Last update June 2012